ALIX is recruited temporarily into HIV-1 budding sites at the end of gag assembly.
Polymerization of Gag on the inner leaflet of the plasma membrane drives the assembly of Human Immunodeficiency Virus 1 (HIV-1).Gag recruits components of the endosomal sorting complexes required for transport (ESCRT) to facilitate membrane fission and virion Custom Sweatshirt release.ESCRT assembly is initiated by recruitment of ALIX and TSG101/ES